You can import simple images, such as camera images or more complex images, such as a light microscope image with overlays, into your ZEN Connect project.

You can use an imported image as a backdrop to navigate the region. You can correlate imported images with sample holder marks, e.g., fiducials or other images through the alignment process. The imported image is displayed according to its position in the Layers View along with any other image in the project.

The metadata of .CZI-images are read natively and are also imported.

  1. You have loaded a ZEN Connect project.
  1. In the ZEN Connect tool in the on Project View , right-click a session and select Add image. Alternatively, for the Import button, select Add image. If you want to add an image from ZEN Data Storage (without downloading it), right click in the Imported Data and select Add image from storage.
  2. You are prompted to select the image you want to add.
  3. If you want to import the image as a background image (at the lowest level of the project structure), activate the Background Image checkbox.
  4. You also have the option to copy the image in a subfolder of the file where the project data is stored before adding it to your project. Activate Copy files.
  5. Click on Open.
  1. The image is imported to your ZEN Connect project.
  2. In the Project View, it is displayed in the tree, subordinated to Imported Data.
  3. If Shuttle & Find-calibration is available for the image and the image is at least in one session in the ZEN Connect project, the image is placed in the Image View according to the Shuttle & Find stage position. Take care that you select the correct sample holder when importing an S&F-calibrated image. The correct sample holder is the same sample holder that was used during acquisition.
  4. If no Shuttle & Find data is available, the image is displayed centrally in the FOV of theImage View.

To add an open image to a project, see Adding an Open Image to the ZEN Connect Project, and to add a dataset, see Adding Datasets when Adding Images.

Additionally, you have the following options to import data in your ZEN Connect project:

Note: If you import an Airyscan image, ZEN Connect displays only the raw data and not the calculated Airyscan. Such images should be processed before you add them to ZEN Connect. If you want to add an unprocessed Airyscan image, a warning will appear asking if you want to continue.

Note for z-stacks: When you use the Global Z slider and are beyond the range of a certain image, only a frame for this image is displayed to show where the image is positioned.