If you see the error message Smart Setup calculation failed, it was not possible for Smart Setup to calculate any proposal. This may be because the filters and light sources available on the system do not allow an image of the dye to be acquired with a good signal strength or with little crosstalk. The channel for this dye or the contrast method cannot therefore be created. In this case, try selecting another, similar dye.

Should the error message be displayed for all dyes that you select, this may be due to one of the following causes:

- no light source has been configured or the light source is switched off.

- no camera has been configured on the system, the camera is not connected or (on some models) has been switched off.

  1. On the Acquisition tab, click Smart Setup.
  2. The Smart Setup dialog opens.
  3. Select the WF button on top of the dialog.
  4. To add a channel, click + Dyes & Contrast Methods.
  5. The Add Dye or Contrast Method dialog opens.
  6. Double-click desired dye or contrast method.
  7. The dye is then adopted directly into the experiment. You have added a channel to your experiment. To add further channels, repeat the previous step.
  8. To return to Smart Setup, close the dialog.
  9. You will now see a graphic overview in the Proposals section. This displays the spectra of the dyes, the expected signal strengths per dye and the spectral crosstalk schematically.
  10. Depending on which dye you have selected and the microscope hardware available, up to three different proposals (Best Signal, Fastest, Best Compromise) are displayed. These differ in terms of signal strength, crosstalk and speed. Select the proposal that best meets the needs of your experiment.
  11. To select a proposal (if there is more than one) for all active configured channels, activate the proposal.
  12. To optimize experiment settings additionally, click on a Motif button. The Automatic button is set as default.
  13. With the Motif buttons you can optimize acquisition parameters and camera settings automatically, either for a high quality image (Quality) or a faster acquisition but reduced image quality (Speed). Find a more detailed description of Motif buttons in Smart Setup dialog.
  14. To optimize experiment settings, adopt the suggestion, and leave Smart Setup, click OK.
  15. The added channels are adopted automatically into the Channels tool.
  16. Click Set Exposure at the top of the Acquisition tab.
  17. The exposure time is now measured for all three channels one after the other. This is adopted into the settings for the channels. Following the measurement of the exposure time, the multi-channel image is acquired automatically and displayed in the Center Screen Area.
  18. To save the experiment together with all the settings, go to the in the Experiment Manager and click .
  19. Options are displayed.
  20. Select the Save entry in the dropdown list.
  1. You have set up the multichannel experiment using Smart Setup, executed it and then saved the configuration. This means that you can repeat the experiment as often as you like using the same settings.