1. Open the MeanROI setup from inside the Dynamics tool.
2. Mean ROI definition opens and snaps of the configured channels are acquired automatically and displayed in the Center Screen Area. The diagrams for each image are displayed to the right of this.
2. Select the Online Ratio tab from the view options and activate live ratio generation.
3. Under Method select the Dual Wavelength entry from the dropdown list.
4. Under Calculation select the Fura-2 340 nm entry from the dropdown list in the numerator of the formula.
5. Under Calculation select the Fura-2 380 nm entry from the dropdown list in the denominator of the formula.
6. A preview of the ratio image, which is calculated according to the ratio settings, is displayed.

1. You have successfully activated the ratio functions and specified the calculation of the ratio.