Rolling ball performs a background correction based on the work of Sternberg, Stanley R. “Biomedical image processing.” Computer 1 (1983): 22-34. It determines the background in an image locally which allows this to be used on unevenly illuminated images as opposed to using a global background value. The size of the ball should be at least as large or exceed the size of the largest object, which is not part of the background.

Parameter

Description

Radius

Sets the radius of the rolling ball creating the background in pixels.

Background Only

Activated: Only creates a background, but does not subtract it.

Smooth

Activated: Smoothes the image before creating the background.

The following parameters are only visible, if you have a CUDA capable GPU.

GPU

Activated: Uses the GPU to perform the rolling ball background correction.

Subsampling

Activated: Applies subsampling to reduce resource consumption of the GPU.