Lightsheet processing allows you to deconvolve, register, and fuse Multiview images.

Parameter

Description

Settings

Allows handling predefined settings (templates). See also General Settings.

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New

Creates a new setting. Enter a name for the setting.

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New from Template

Creates a new setting based on an existing template.

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Rename

Renames the setting.

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Save

Saves a modified setting under the current name. An asterisk indicates the modified state.

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Save As

Saves the current setting under a new name.

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Import

Imports an existing setting.

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Export

Exports the current setting.

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Delete

Deletes the current setting.

Deconvolution

Activated: Allows to deconvolve Lightsheet data (single views) of the following formats:

  • Multiview experiments before registration and fusion (single or dual side illumination):
    Deconvolution is performed for each frame from all z-stacks and all views. The process uses a point spread function (PSF) valid for single view images.
  • Multiview experiments after registration and fusion:
    Deconvolution is performed on the fused dataset, hence only one z-stack needs to be processed. It uses a star-like point spread function (PSF) computed from the fused Multiview image.

For more information, see Deconvolution (adjustable).

Multiview

Activated: Allows registration, fusion, and deconvolution of Multiview images.

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Registration options

Allows to select the alignment method:

The following datasets can be processed with this function:

  • Multiview datasets, single side illumination
  • Multiview datasets, dual side illumination, Dual Side without Fusion
  • Multiview datasets, dual side illumination, Dual Side with Fusion
  • Multiview datasets with Deconvolution
  • Multiview datasets without Deconvolution

The Registration generates the transformation matrices so that all views are matched to overlay. As a result, a dataset is provided in which all z-stacks of all views should already largely overlay. The reference at which registrations starts is always View 1 of the dataset. This image can be used for control. If one or more views do not have the same alignment, registration was not successful, and the parameters need to be adjusted. The Registration must always be done before the Fusion of the data.