This section of the Coarse Z-Stack Alignment setup contains the main controls to align the z-planes of your stack manually.

Parameter

Description

Speed

Selects the speed/ number of pixels by which the z-planes are shifted per click.

Shifts the z-planes by 1 pixel per click.

Shifts the z-planes by 10 pixels per click.

Shifts the z-planes by 100 pixels per click.

Shifts the z-planes by 1000 pixels per click.

Arrow Buttons

Shifts the next and all following z-planes in the corresponding direction.

Visibility Weight slider

Changes the color intensity of the current plane and the following z-planes in the Image View. In extreme slider positions, only the current z-plane or only the following z-planes are displayed.

Channels

Only available for images with multiple channels.

Selects the channel that is displayed in the Image View. The same shift is applied to all channels of the image.

Shift List

Displays a list with all the shifts in x and y direction that you defined in the setup.