1. You are on the Acquisition tab in the Tiles tool.
  1. Open the Positions section.
  2. Start the Live mode to use the stage to locate a position that you want to acquire.
  3. The X and Y coordinates of the current position are displayed in the Current X/Y display fields.
  4. Bring the specimen into focus using the focus drive.
  5. Click .
  6. The current position is added to your experiment.
  7. If you are close to a position that you added previously the software will ask you if you really want to add another position at this location (the threshold for this lies within a circle whose radius is less than half the approximate width of the cameras visible field).
  8. To add further positions, move the stage to another position on the sample and repeat the previous steps.
  9. The added positions are shown in the list in the Single Positions section with their X, Y and Z-coordinates.
  10. Save the experiment. To do this, in the Experiment Manager click and select Save As. Enter a name for the experiment in the input field (e.g. Simple Tile Experiment).
  11. Click Start Experiment.
  12. The Positions experiment is acquired.
  13. The individual positions are displayed in the acquired file as scenes and can be selected using the Scene slider on the Dimensions tab. If you deactivate the Scene checkbox, all positions are displayed simultaneously as an overview.
  1. You have successfully set up and acquired a Positions experiment.

Z values

To ensure that the individual Z values of the positions are taken into account the software automatically selects the most appropriate focus strategy when the checkbox Tiles is activated. For the experiment described here no further modification needs to be made. If you want to acquire all positions at the same Z-position, then you have to select None from the dropdown list in the Focus Strategy tool. The individual Z-positions are then ignored and the current Z-position at the time the experiment is started is used for all positions.

Shortcut

You can also add a single position at the current stage position by pressing the F10 button on your keyboard.