1. You have created a Dynamics Profiler document where you have set the Time Resolution to ωr in the third step of the wizard.
  2. This Dynamics Profiler document is open in the Correlation view.
  3. The objective for your spot experiment must be the same as the objective you use for the ωr calibration.
  1. In the view options, open the ωr Calibration tab.
  2. From the Dye dropdown list, select which dye you want to use for the calibration.
  3. The value for Diffusion Coefficient is updated automatically.
  4. If you know the Diffusion Coefficient for your experiment, adapt the value in the input field accordingly.
  5. Click Calibrate.
  6. A file browser opens.
  7. Select the folder where you want to save the calibration file and enter a file name.
  8. Click Save.
  9. The custom ωr calibration is created and the file is saved.