1. You have switched on and configured your microscope system including all components.
2. You have set up a new experiment, at least defined one channel and adjusted focus and exposure time correctly, see Setting Up a New Experiment.
3. You are on the Acquisition tab.

1. In the Acquisition dimensions section activate the Z-Stack checkbox.
   
2. The All Tracks per Slice selection box is displayed.
3. The Z-Stack tool appears in the Multidimensional Acquisition tool group.
2. Use the All Tracks per Slice option or select the Full Z-Stack per Track option in the selection box.
3. Click on the blue bar to open the Z-Stack tool.

1. You have successfully completed the general preparations. You can now set up z-stack experiments automatically or manually.