Before starting the experiment itself, we recommend tthat you first take a reference image for the shading correction. This image will be used later for processing the panorama image.

  1. Move the sample to an empty field.

  2. Ensure that the field of view is absolutely free of any structures (dirt); as this would cause errors in the images acquired later. If necessary, slightly de-focus the sample to suppress these structures.

  3. Click on the Snap button to acquire this position as reference image.
  4. Save the image and let it open in the background.
  1. You have successfully acquired a reference image for the shading correction.