In this step you will select the image to be processed and load it as an input image for deconvolution.

  1. On the Processing tab, select the Deconvolution (adjustable) method.
  2. In the Input tool, select the image that you want to deconvolve. If Set Input Automatically is activated, the currently active image has been loaded as input image automatically. Since this means, that the output image is then also automatically selected as input, it is recommended to deactivate this checkbox.
  3. Select the desired input image.

If a warning appears at this point, it is likely that parameters required for deconvolution are missing from the image. You can subsequently enter or change these values in the Parameters tool > PSF settings tab > Microscope parameters.