Youvan et al., Biotechnology et alia 3, 1 (1997)

Fc = F - D*(FD/DD) - A*(FA/AA)

Displays the Fc image with intensities converted from the FRET index calculated for each pixel using the Youvan method. This method assumes that the signal recorded in the FRET channel is the sum of real FRET signal overlaid by donor crosstalk and acceptor signal induced by direct (donor) excitation. There is no correction for donor and acceptor concentration levels and as a result the FRET values tend to be higher for areas with higher intensities.