On this tab, you can manually set the threshold for image analysis using the slider or input fields. Alternatively, the threshold can be set by defining and enabling a background region in the FRET tab.

The values for the thresholds are either displayed as grey value levels (Raw Data) or Normalized data, which is normalized to the value 1.

If you have activated Subtract Background Using Thresholds on the Settings tab, the background values are subtracted before the image analysis is performed.