The processing of large y-stack images or long time series can take some time. During processing we recommend that you do not perform any other complex actions in ZEN or in other programs on the computer, to avoid increasing the processing time unnecessarily.

In this step you perform deconvolution. You can then compare the resulting image to the input image and details relating to the processing procedure are visible.

  1. Go to the Deconvolution tab
  2. Click Apply on the top of the Processing tab.
  3. The progress is displayed in the Convergence History graph, in which the gradual improvement is plotted against the number of iterations.
  4. Save the resulting image under a meaningful name.
  5. Click on the Splitter Mode button in the document bar. This creates a multi-image (split image).
  6. The currently active image is loaded automatically into the multi-image. Now drag the input image from the document gallery in the Right Tool Area into the split document.
  7. On the Display tab, change and adjust the display as desired, e.g. Best Fit with 0.01 and for black and white value with a Gamma of 0.8.
  8. Both images are adjusted simultaneously.
  9. If on the Split Display tab Synchronize Dimensions is activated, you can now zoom synchronously into the images (mouse wheel) and, with the mouse wheel held down, move the image content as desired to focus on the regions of interest.
  10. To create an image of the desired view click on New Image from > Current View > Create.
  11. This creates a new output image document with both images shown side by side.
  1. You have successfully performed deconvolution, observed the processing procedure, and created an output image to compare the deconvolved with the input image.