Parameter

Description

Z-Stack

Activated: Stores all acquired images in memory and calculates the EDF image based on all images after each individual acquisition. Note that the more images you acquire, the more time it takes to calculate the EDF.

Deactivated: Calculates the EDF image based on the last acquired image and the previously calculated EDF image. Thus this mode is only additive and does not store all acquired individual images nor does it recalculate the entire image stack.

Quality

Selects the quality level that you want the function to work with.

Registration Method

Selects the method (or combination of methods) that is used to align the images.

-

Translation

The neighboring sections of the z-stack image are shifted in relation to each other in x and y direction.

-

Rotation

The neighboring sections of the z-stack image are rotated in relation to each other.

-

Iso Scaling

The magnification is adjusted from section to section.

-

Skew Scaling

The neighboring sections of the z-stack image are corrected for skewness/shearing.

-

Affine

The neighboring sections of the z-stack image are shifted in x and y direction, rotated and the magnification is adjusted from section to section.

Interpolation

Selects the interpolation that is performed if a pixel is calculated from several individual pixels.

-

Nearest Neighbor

The output pixel is given the gray value of the input pixel that is closest to it.

-

Linear

The output pixel is given the gray value resulting from the linear combination of the input pixels closest to it.

-

Cubic

The output pixel is given the gray value resulting from a polynomial function of the input pixels closest to it.

Mode

-

Timer

Acquires an EDF (Extended Depth of Focus) image automatically after a defined time interval.

-

F12 Key

Acquires an EDF image when you press F12 key.

Interval

Only active if Timer is selected.
Sets an interval (in sec.) after which the individual images are acquired.

Start

Starts the acquisition of an image series.

-

Pause/Continue

Pauses/Continues the acquisition.

-

Stop

Stops the acquisition.