This method accepts ApoTome raw data only. The settings are similar to the ones on the ApoTome tab (view option for ApoTome images). The function is also available for batch processing, which makes it easy to convert a series of ApoTome RAW data images into deconvolved images.

Parameter

Description

Display Mode

ApoTome images are acquired as raw data. The Display Mode sets how the image is calculated and displayed.

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Optical sectioning

The displayed output image is calculated/rendered by eliminating the excitation and emission light that originates in regions outside of the focal plane.

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Conventional fluorescence

The displayed output image is calculated/rendered like a conventional fluorescence image.

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Raw data

Displays the raw data as output image and disables all other parameters of the function.

Correction

Applies stripe artifact correction to the resulting image. It attempts to remove stripe artifacts which may be caused by bleaching of the sample during acquisition or by slight deviations in the grid phase position.

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No correction

No correction is applied to the image.

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Local Bleaching

Corrects the bleaching for each pixel individually (default setting). This is usually the best method.

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Global Bleaching

Corrects bleaching by means of global bleaching correction, which is applied equally to the entire image.

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Phase Errors

Corrects phase errors in the image without additional bleaching correction.

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Phase Errors and Global Bleaching

Corrects phase errors in the image with additional global bleaching.

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Phase Errors and Local Bleaching

Corrects phase errors in the image with additional local bleaching.

Fourier Filter

The Fourier filter attempts to remove residual stripes.

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Off

Uses no Fourier filter to remove stripes.

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Weak

Uses a weak Fourier filter to remove stripes.

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Medium

Uses a medium Fourier filter to remove stripes.

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Strong

Uses a strong Fourier filter to remove stripes.

Normalization

Here you can select how the gray/color values that exceed or fall short of the value range should be dealt with. If you use this method with Direct Processing, only the Clip method is available and preselected.

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Clip

Automatically sets the gray levels that exceed or fall short of the predefined gray value range to the lowest or highest gray value (black or white). The effect corresponds to underexposure or overexposure. In certain circumstances some information may therefore be lost.

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Automatic

Normalizes the gray values automatically to the available gray value range.