Correlation

Transform

Here you select which Z-Stack will be transformed. During transformation, a pixel in the overlay image of the Z-Stack is calculated by using pixels of the two original images that shall be overlaid/merged.

Interpolation

Here you can select one of the following interpolation methods:

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Nearest Neighbor

The gray value of the resulting pixel in the overlay image is made of a pixel which is located next. This interpolation method is very fast.

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Linear

The resulting or calculated pixel in the overlay image is assigned to a gray value, which is the result of a linear combination of gray values derive from pixels located nearby (in the original image).

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Cubic

The calculated pixel in the overlay image is assigned to a gray value, which is calculated by means of a polynomial function using gray values of pixels in the original images; these pixels are located nearby the calculated pixel.

Mode

Here you can choose an algorithm mode:

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3-Points

If selected, this mode enables you to set 6 correlation points after clicking on the Set Points button (3 points in each Z-Stack in each container).

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4-Points

If selected, this mode enables you to set 8 correlation points after clicking on the Set Points button (4 points in each Z-Stack), 3 points in the first z-section, the last point in the last section.

Correlation Points

If you click on the Set Points buttons, you can set the correlation points.

The number of correlation points is according to the selected algorithm. The cursor will change to a pipette symbol. Simply click in the image to set the points. Start with setting the first three points in the left container then set the corresponding correlation points in the right container. If a correlation point is set, a check mark icon will appear in front of the corresponding point.

When you select the 4-Points-Algorithm, the display will move automatically to the last image of the Z-Stack. Set the fourth correlation point in both containers. Make sure that the positions in both Z-Stacks are identical. After you have set all correlation points, the cursor will be changed backwards from the pipette to the arrow.

Reset deletes all correlation points in the image.

Create correlated Z-Stack

If you click on this button, the correlated Z-Stack will be generated and opened in a new image container.