Use this function to subtract background values from the measurement values. A background correction will allow you to make a better comparison of the magnitude of any fluorescent intensity changes observed over the time course of an experiment. Determine the background value with the help of a Background ROI or define a fixed value. Note that the background correction for ROI is only available if there are at least two ROI defined in the image!
If the ratio calculations are enabled, the background correction parameters are defined on the Ratio tab. The background correction values on MeanROI tab are disabled, i.e. not used in this case.

Defining a Background ROI

  1. You are in the MeanROI view or MeanROI setup.
  1. At the desired time point of the time series, draw a ROI into a part of the image that contains only background signal in all channels.
  2. Go to the MeanROI tab > Background Correction section and activate the radio button ROI. Note that this function is only available if two or more ROIs are present.
  3. In the drop down, select the ROI-ID of the ROI that you defined in the first step.
  4. To edit the Background ROI, simply draw a new ROI and select it from the dropdown list.
  1. You have successfully defined a Background ROI. The mean intensity of the background ROI is subtracted from the measured values of the ROIs in a channel- and time-point-specific manner. The corrected values are adopted into all diagrams and tables.

Defining a fixed background value

  1. You are in the MeanROI View or MeanROI Setup.
  1. On the MeanROI tab in the Background Correction section select the Constant option.
  2. The associated input field is activated.
  3. Enter a fixed background value into the Constant input field.
  4. Press Enter to update the measurements.
  1. The defined background value is subtracted from all measured values of the ROIs in a time-point-specific manner.