In this topic we will show you how to extract individual fluorescence channel images of a multichannel image.

1. You have acquired or opened a multichannel image.
   

1. Select the Processing tab. Open the Method tool and select under Utilities the entry Create Image Subset.
2. In the section Image Parameters, open the Input tool and select the multichannel image as Input image.
3. In the section Method Parameters, open the Parameter tool and the select the entry Channels. Deactivate the channels you do not want in the extracted image (e.g. the red and the blue fluorescence channel).
   
4. Click on the Apply button.

1. In the example image, you have extracted the green fluorescence channel image of the multi-channel image. Activate the corresponding channel and deactivate the other channels to extract further individual fluorescence images. You can now save the extracted sub images as separate files.