1. You have licensed the Spatial Biology toolkit and activated it in Tools > Toolkit Manager.
  2. You have a reference scan profile that was set up by an expert user.
  1. In the Magazine view, click Customize Reference Scan Profile.
  2. The first step of the Customize Reference Scan Profile wizard opens.
  3. For Reference Scan Profile, select the scan profile you want to adapt, and define a Batch Name.
  4. The scan profile is selected.
  5. Click Next.
  6. The next step opens.
  7. Check all the listed channels from the original scan profile and activate the checkbox for all channels you want to use. If necessary, adapt the focus strategy and marker.
  8. You have reviewed and selected channels for the adapted profile.
  9. Click Next.
  10. The next step opens.
  11. Select the reference slide with the two drop-downs and click Onboard Slide. Make sure you select a slide that is typical for the current batch of slides you want to scan.
  12. The reference slide is loaded and an overview experiment is executed, including the tissue detection.
  13. The acquired image is displayed in the Center Screen Area.
  14. Use the controls to define the exposure times for the individual channels. Click Auto Exposure to start an exposure time measurement for all channels, or click Auto for a single table entry to start a measurement for that respective channel. Alternatively, set the exposure time manually with the input field in the table.
  15. The exposure times are set.
  16. Click Snap to acquire a snap, or click Live to start a live acquisition.
  17. An image is displayed in the Center Screen Area.
  18. Check the image to see if the exposure times are set correctly. If necessary, repeat the previous steps to further adapt the exposure times.
  19. You have checked the updated exposure times.
  20. To save the values when executing multiple exposure time measurements, click Record Exposure Times before a new measurement or exposure time change.
  21. The exposure times for each marker are saved and displayed in the table of the Recorded Exposure Times section.
  22. When you have set suitable exposure times for the new batch of slides, click Finish.
  23. All changes are saved and the wizard closes.
  24. The reference scan profile is adapted and a copy of the original scan profile is created.