In this topic we will show you how to create an extended depth of focus (EDF) image of a Z-Stack image. The focus planes of all the z-positions will be calculated to one EDF image.

1. You have acquired or opened a z-stack image.

1. On the Processing tab, open the Method tool and select under Sharpen the entry Extended Depth of Focus.
2. In the Image Parameters section, open the Input tool and select the Z-Stack image as input image.
   
3. In the Method Parameters section, open the Parameters tool.
4. Select Wavelets of the Method drop-down list.
5. Select High of the Z-Stack Alignment drop-down list.
6. Click on the Apply button.

1. All focus planes of the Z-Stack image are now calculated to one EDF image.