1. You have created a new image analysis setting with the Segment region classes independently method and opened the Image Analysis Wizard, see Creating a New Image Analysis Setting.

1. In the Classes step, click on Class 2 in the list and enter DAPI Individual Nuclei in the Name input field.
2. Click on Classes1 in the list and enter DAPI All Nuclei in the Name input field.
   
3. Click Next.