ZEISS Microscopy Knowledge Base
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ZEISS Microscopy Knowledge Base

AI-based methods

AI Semantic Segmentation

Utilizes machine learning algorithms to classify regions based on a trained Intellesis (Link) model (based on random forest pixel classification) or an imported deep learning network, e.g., ONNX models or models trained on arivis Cloud.

Use case: Labeling image regions defined by classes in a previously trained model.

For information on labeling/annotating images to train a model on arivis Cloud, see Annotation of Images for Model Training on arivis Cloud.

For general information about arivis Cloud, see arivis Cloud Overview.

Microscopic image of curved tubular structures outlined in blue and red over a field of multicolored circular dots

Post-processing tools in the 2D Toolkit in ZEN and ZEN core allow further refinement of semantic segmentation results, such as fill holes / binary operations and separation algorithms such as watershed.

AI Instance Segmentation

This method leverages deep learning models for distinguishing and separating individual objects, even when they touch or overlap.

Use case: Provide precise identification of objects in complex images.

Cluster of irregular gray blobs outlined with thin multicolored contours on a gray background

Intellesis Trainable Segmentation

Intellesis Trainable Segmentation, part of the AI Toolkit license, is a powerful machine learning tool integrated into the ZEN and ZEN core platforms, enabling you to perform trainable class segmentation.

Use case: Classify image regions based on specific features, such as intensity and texture by training the models directly within the software.

The Intellesis Training UI provides an intuitive interface for creating and refining models using conventional machine learning techniques, such as random forests. You can then apply these models to classify pixels into predefined classes, facilitating accurate segmentation tailored to the unique characteristics of a dataset.

Intellesis UI with organoid image, colored annotations and right panel labels Organoid image Annotations Predictions
Intellesis Training UI in ZEN
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