Physiology (Dynamics)
This module enables the analysis of physiological time series data with Ca2+calibration, including mean ROI measurement. It supports imaging with single wavelength (e.g. Fluo-4) and dual wavelength dyes (e.g. Fura-2) allows ratio calculations and flexible charting and image display as well as data table display with data export functionality. It also offers the use of definable switches for online annotations, changing of acquisition speed and freely configurable TTL triggers. Some functionality is generally available, for the full set of features you need the dedicated license for the module, see Licensing and Functionalities of Physiology.
See also
Licensing and Functionalities of Physiology
Some basic functionality for physiology experiments is generally available in the software, but the full Physiology (Dynamics) functionality requires a license.
Basic functionality
The basic functionality is available for time series images and time series with z-stack images opened in the software (excluded ZEN lite), or multi-positions (scenes) where a full time series is collected sequentially at each position (Full time series per tile Region). Note that for acquiring time series images, you also need the license for the Time Series module. The functionality generally available in ZEN includes:
- Using the MeanROI offline functions to specify user-defined measurement regions (ROIs) after acquisition of your time lapse experiment and analyze their time-dependent changes in intensity.
- The functionality to display the intensity curves in charts or export the values in the form of tables.
- Definable switches for online annotations and change of acquisition speed. Pausing and refocusing are possible via a live camera view.
Licensed Functionality
If you have licensed this functionality and activated it under Tools > Toolkit Manager, the additional functionality includes:
- The option to calculate online/offline (during/post acquisition) ratios and display a ratio image.
- Additional display layouts and analysis functions (ROI tracing etc.).
Opening the MeanROI View
- You have acquired a time series experiment with one or more channels. The experiment is open and the first time point is displayed in the 2D view.
- Select the MeanROI tab from the image view tabs in the Center Screen Area.
- The MeanROI view opens.
- You are now prepared to start working with the MeanROI view. The following chapters will show you the first steps.