This module enables the analysis of physiological time series data with Ca2+calibration, including mean ROI measurement. It supports imaging with single wavelength (e.g. Fluo-4) and dual wavelength dyes (e.g. Fura-2) allows ratio calculations and flexible charting and image display as well as data table display with data export functionality. It also offers the use of definable switches for online annotations, changing of acquisition speed and freely configurable TTL triggers. Some functionality is generally available, for the full set of features you need the dedicated license for the module, see Licensing and Functionalities of Physiology.