With the ZEN Connect 3D view you can display and align two z-stacks as 3D volumes. This alignment mode allows a translation of the volumes in x, y and z.

  1. You have opened two z-stack images in the ZEN Connect 3D view or directly in 3D alignment mode, see Opening Images in the ZEN Connect 3D View.
  1. In the Connect 3D tab, click the button for the volume you want to align, e.g. Align Volume 1. If you opened the 3D view directly in alignment mode, one volume is already selected for alignment.
  2. You are in alignment mode and the Alignment tab is displayed.
  3. To mirror the selected image, go to the Alignment tab and click to mirror the image horizontally, or click to mirror it vertically.
  4. In one of the 2D image views, shift the volume via drag and drop and align it with the second volume as reference.
  5. The changes are automatically updated in all views, including the 3D view.
  6. If you are satisfied with your alignment, go to the Alignment tab and click Apply.
  7. Your alignment changes are saved in the ZEN Connect project.