Using Direct Processing with Airyscan Processing
- This function is only available if an Airyscan detector is available.
- If you are using Direct Processing on different computers, you have connected acquisition and processing computer, see Connecting Acquisition Computer and Processing Computer.
- To ensure that the processing computer reads incoming files and starts the processing, on the Applications tab, in the Direct Processing tool, you have clicked Start Receiving. This is usually active by default.
- On the Acquisition tab, you have set up your experiment for image acquisition.
- On the Acquisition tab, Direct Processing is activated. This activates the Auto Save tool as well.
- Depending on your settings, you have defined the folder where the acquired images are stored in the Direct Processing or the Auto Save tool. Use a folder to which the processing computer has access. For information about sharing a folder, see Sharing a Folder for Direct Processing.
- On the Acquisition tab, open the Direct Processing tool.
- If no Direct Processing settings were made before for the current experiment, a particular processing function is already preselected depending on your microscope, channel settings and licenses.
- In the Processing Function drop-down list, select Airyscan Processing.
Note: If you uncheck the Auto Filter checkbox and activate Adjust per Channel, you can set the Super Resolution parameter in a channel specific manner. - Select the desired settings for Airyscan Processing. For details how to use this function, see Airyscan Processing. Ideally, you have already checked the best parameters beforehand, using a sample image acquired under the same conditions as set up for the experiment.
- Click Start Experiment to run the experiment.
Note: You can pause the processing. If you stop the experiment, requests that have been sent earlier by the acquisition computer are not processed. However, already processed images will be retained. - The images are stored in the folder you have defined in the Auto Save or Direct Processing tool. When you abort the acquisition, the remote Airyscan processing will not take place.
- The processing computer reads incoming files and starts the Airyscan processing. The path to the selected folder, the currently processed image as well as the images to be processed are displayed in the Direct Processing tool. The processed image is saved to the same folder specified in the Direct Processing tool. If the image name already exists in this folder, the new file is saved under a new name <oldName>-02.czi.
- To cancel the processing, click Cancel Processing.
- Once processing is finished, you are notified on the acquisition PC and can open and view the acquired image as well as the processed images. This should be done on the processing computer so that you immediately can start a new experiment on the acquisition computer. However, you can also automatically open the processed image on the acquisition PC with the respective setting on the Direct Processing Tool on Acquisition Tab.
- When you open the image in the Image View, information about the executed Airyscan processing is available on the Info view tab. Additionally, general information about Direct Processing (e.g. the duration) is also available on the Info view tab of the processed image.
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