The additional options for the tiles module allow to set up several options for image acquisition and additional information. The tiles options dialog can be found in the menu bar under Tools > Options > Acquisition > Tiles & Positions.
Note: For the changes to be effective you might need to close and reopen the advanced setup viewer.

Parameter

Description

Automatically Start Live Mode in the Advanced Setup View

Activated: Automatically starts the Live mode in the Center Screen Area if you klick in the Acquisition tab in the Tiles tool on the Advanced Setup button.

Uncheck this option to prevent unnecessary specimen bleaching. The default is not activated.

Automatic Snap by Clicking the Live Navigator Buttons

Activated: Acquires an image if you click on one of the frame's blue arrow icons. The Live Navigator tool moves one frame width in the relevant direction. You can create tile images of your sample easily in this way.

Enable Stage Movement with Live Navigator

In the Live navigator tool the current stage position including the live image is shown as a frame outlined in blue. To move the frame, double-click on the position to which you want to move it. Alternatively, place the mouse cursor over the blue frame, press and hold the left mouse button and drag the live navigator to the desired location.

Activated: Allows you to move the Live Navigator tool by dragging it to a new location.

Show Stage and Focus Backlash Correction Setting in the Options

Activated: In the Tiles option, the setting to switch the backlash correction on or off is shown. Per default it is hidden.

Delimiter for CSV Export/Import

Specifies the delimiter for a CSV export or import. Select Comma (default), Semicolon or Tab.

Ask Whether Support Points/Positions Should be Overwritten

When the support points and/or positions are determined by a software autofocus run the existing points can be overwritten with the new Z values.

Activated: Shows a message box asking if the points should be overwritten if there is a autofocus Z value.

Focus Surface Outlier Determination

Ignores support points that are significantly outside the interpolated focus surface.

You have the following setting options available:

-

Maximum Interpolation Degree for Outlier Detection

This value can be 0 or 1. If 1 then a linear fit is used to detect the outlier support points. This is the default. If 0 a simple average value is used to detect outliers.

-

Threshold in Terms of the Standard Deviation (Sigma)

This parameter defines a threshold value to determine which of the support points are outliers from the fitting process. This is defined by the standard deviation (sigma value) set in the spin box. Support points not meeting this criteria are subsequently ignored when the focus surface is determined.

Delay Time After Stage Movements

Defines a delay period which is used for all stage movements in a tiles and position experiment or movements controlled in the advanced tile setup. The delay helps prevent movement in samples where, for example, a large volume of liquid is present in the sample holder. It can be used with the stage speed and acceleration options to optimise experiments with this type of sample.

Binning Compensation of Exposure Time in Preview Scans

Defines the power to which the binning ratio is modified to automatically determine the exposure time value used for a preview scan were the binning setting between the experiment and preview scan differs. The default value is 2.0 i.e. quadratic. Thus, for example the exposure time would be reduced by a factor of four if the experiment binning is 1x1 and the preview scan binning is 2x2. The value can be varied between 1.0 and 2.0 in steps of 0.1.

Live Image in Sample Carrier Calibration Wizard (relevant only for systems with camera)

-

Use Imaging Device from Selected Channel with "Acquisition" Settings

Activated: Default setting for the live image that allows navigation and focus interaction during the carrier calibration wizard.

-

Use Active Camera with "Locate" Settings

This option is only relevant for systems with a wide field (camera based) detector.
Activated: Allows you to alternatively apply locate camera settings for use in the carrier calibration wizard (live image). By default the experiment settings for the currently selected channel/track will be used.