Processing Not Possible on Acquisition Computer

For Lightfield 4D in Direct Processing, you cannot process the image on the acquisition computer. You must connect your acquisition computer to a dedicated processing computer running ZEN desk, see Connecting Acquisition Computer and Processing Computer

Lightfield 4D can be used in Direct Processing. For general information, see Direct Processing.

  1. To ensure that the processing computer reads incoming files and starts the processing, on the Applications tab, in the Direct Processing tool, you have clicked Start Receiving. This is usually active by default.
  2. On the Acquisition tab, Direct Processing is activated. This activates the Auto Save tool as well.
  3. Depending on your settings, you have defined the folder where the acquired images are stored in the Direct Processing or the Auto Save tool. Use a folder to which the processing computer has access. For information about sharing a folder, see Sharing a Folder for Direct Processing.
  4. If you want to execute an experiment with high-speed acquisition, the path for Auto Save has to be a location on the SSD of the acquisition computer! At the same time, the path for the output folder of Direct Processing should be set to a location on the processing computer (see steps further below). Always use folders where both computers have access to.
  5. On the Acquisition tab, you have set up your experiment for Lightfield 4D acquisition, see also Setting Up and Starting a Lightfield 4D Experiment.
  1. On the Acquisition tab, open the Direct Processing tool.
  2. The tool parameters are displayed. In the processing pipeline, the first block is selected automatically
  3. If the function is not preselected, go to the Processing Function dropdown list and select Lightfield 4D.
  4. The parameters are displayed, see Lightfield 4D Processing.
  5. To adjust the parameters individually for each channel of a multi-channel image, activate Adjust per Channel.
  6. The tool displays all channels as a list and the currently selected channel is highlighted. For each channel you can use a dropdown to select whether to process it or to skip it (the channel is not processed and not part of the output image).
  7. Define the settings for the parameters.
  8. The parameters are set.
  9. If you set up an experiment with high speed, open the Output section and deactivate Use Output Folder from Auto-Save.
  10. The location setting gets editable.
  11. Click .
  12. A file browser opens.
  13. Select a folder on the processing computer and click Select Folder.
  14. The new output folder is set.
  15. Click Start Experiment to run the experiment. Note: You can pause the processing. If you stop the experiment, requests that have been sent earlier by the acquisition computer are not processed. However, already processed images will be retained.
  16. The images are stored in the folder you have defined in the Auto Save or Direct Processing tool. When you abort the acquisition, the remote processing will not take place. In case you have set up several processing functions, only the acquired image and the final output image are stored.
  17. The processing computer reads incoming files and starts the processing. The path to the selected folder, the currently processed image as well as the images to be processed are displayed in the Direct Processing tool. The processed image is saved to the same folder specified in the Direct Processing tool. If the image name already exists in this folder, the new file is saved under a new name <oldName>-02.czi.
  18. To cancel the processing on the processing computer, on the Applications tab, in the Direct Processing tool, click Cancel Processing.
  1. Once processing is finished, you are notified on the acquisition PC and can open and view the acquired image as well as the processed image. This should be done on the processing computer, so that you can immediately start a new experiment on the acquisition computer. However, you can also automatically open the processed image on the acquisition PC with the respective setting in the Direct Processing tool on the Acquisition tab.
  2. Information about Direct Processing (e.g. the duration) is available on the Info view tab of the processed image.