Parameter

Description

Options

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New

Creates a new Automated Photomanipulation setting. Enter a name for the setting.

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Rename

Renames the setting.

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Save

Saves the current setting.

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Save as

Saves the current setting under a new name. Enter a name for the setting.

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Import

Imports an existing setting.
The setting is shown in the setting drop-down list.

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Export

Exports the current setting.

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Delete

Deletes the current setting.

Experiment

Selects the experiment setup to execute the photomanipulation including Tiles, Time Series and Bleaching.

Analysis

Selects the image analysis setting used to analyze the multi-position image to define the Experiment Regions for photomanipulation.

Class

Defines the class/parameter to find the photomanipulation experiment regions.
Important: The classes in the analysis and their corresponding channel names must exactly fit to the channel names in the experiment.

Sorting Feature

Defines the acquisition order of the Experiment Regions for photomanipulation depending on the selected features in the analysis (e.g. ID, mean intensity in channel x, perimeter, etc.).

Region Type

Defines the shape of the photomanipulation area: Polygon, Circle, Rectangle, Custom Circle/Rectangle (Size and Offset adjustable), Circle, and Rectangle are bounding/containing the detected photomanipulation regions.

Max. Number

Defines how many photomanipulation ROIs per position should be executed.

Offset

Only visible if Custom Circle or Custom Rectangle is selected.

Defines the Experiment Regions offset of the Custom Circle/Rectangle in X and Y direction.

Size

Only visible if Custom Circle or Custom Rectangle is selected.

Defines the Experiment Regions size of the Custom Circle/Rectangle in width and height.

Output Folder

Selects the folder where the experiment results are saved. A subfolder will automatically be created for each run of an Automated Photomanipulation experiment, including the pre-bleach image for Experiment Regions identification, the table of all Experiment Regions, and the experiment results.

Start

Starts the Automated Photomanipulation. The experiment can be stopped with the Stop button in the Acquisition tab. Click on Stop to stop the running Automated Photomanipulation workflow.