This function merges the image done with the lightsheet from the right side with the image of the left side. It can be used for the following datasets acquired with Dual Side illumination:

  • Image (Snap function)
  • Time Series
  • Z-Stacks
  • Combinations of time series and z-stacks
  • Multiview experiments in combination with time series

Parameter

Description

Settings

Allows handling predefined settings (templates). See also General Settings.

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New

Creates a new setting. Enter a name for the setting.

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New from Template

Creates a new setting based on an existing template.

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Rename

Renames the setting.

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Save

Saves a modified setting under the current name. An asterisk indicates the modified state.

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Save As

Saves the current setting under a new name.

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Import

Imports an existing setting.

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Export

Exports the current setting.

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Delete

Deletes the current setting.

Mean

The average intensity for each pixel is calculated using the values of both illumination sides of the fusion.

DFT

Discrete Fourier Transform fusion according to publication. Fine structures and details are more visible after the fusion, which might be worsened when using Mean Fusion. However, this process can create noticeable artifacts in the resulting image.

Maximum

The intensity of each pixel of both illumination images is compared and the value with the higher intensity is used for the resulting image of the fusion.

Fusion Subset in X

Only available for Mean or Maximum fusion.
Selects which area of the left and the right illumination image should be used for fusion.

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Current View

Displays the selected view of a Multiview image in the 2D tab which is used for fusion subset in x.
Note: The position of the sliders Left and Right as well as the Blending value have to be defined for each view individually.

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Left

Selects the area of the left illumination side which is used for fusion. The spin box shows the cut-off x pixel value. The cutoff is shown in the 2D view with a red line.
Note: It is best to set the illumination slider of the Dimensions view control to 1 to see the left-side illuminated image.

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Right

Selects the area of the right illumination side which is used for fusion. The spin box shows the cut-off pixel value of the y axis. The cutoff is shown in the 2D view with a blue line.
Note: It is best to set the illumination slider of the Dimensions view control to 2 to see the right-side illuminated image.

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Blending

Defines the number of pixels used within an existing overlap between left and right illumination to crossfade both illuminations sides.
Note: To avoid a visible change in intensity along the two cut off lines for Mean Fusion with Fusion Subset in X, an overlap of both illumination sides should remain in the center of the image.