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ZEISS Microscopy Knowledge Base

Shuttle & Find View

Besides the Shuttle & Find tool in the Left Tool Area, the S&F (Shuttle & Find) view is visible in the Center Screen Area of the ZEN software. If the S&F view is selected, the S&F tab and S&F Correlation tab will appear as specific view options under the image area.

ZEN S&F split view with two images labeled Snap-15.czi and Snap-17.czi, left image shows red dashed ROI box.
Shuttle & Find View

S&F Correlation Tab

Here you find all functions to overlay (correlate) two images.

S&F Correlation panel with Transform Left/Right buttons, Interpolation Nearest Neighbor, Set correlation points button
S&F Correlation tab

Parameter

Description

Transform

Here you select which image will be transformed. Choose via the Left Image/Right Image buttons, which image should be transformed in the other. During transformation, a pixel in the overlay image is calculated by using pixels of the two original images that shall be overlaid/merged.

Interpolation

Here you can select one of the following interpolation methods:

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Nearest Neighbor

The gray value of the resulting pixel in the overlay image is made of a pixel which is located next. This interpolation method is very fast.

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Linear

The resulting or calculated pixel in the overlay image is assigned to a gray value, which is the result of a linear combination of gray values derive from pixels located nearby (in the original image).

-

Cubic

The calculated pixel in the overlay image is assigned to a gray value, which is calculated by means of a polynomial function using gray values of pixels in the original images; these pixels are located nearby the calculated pixel.

Mirror image

Here you can mirror the image horizontally or vertically. Therefore simply click on the corresponding button.

Mirroring an image is necessary, when the loaded image shows a different orientation than the live image.

Show Correlation

Activated: Opens the correlated image in a new image document/new container.

Set correlation points

Enables you to set 6 points (3 points in each image) as correlation markers in a row, see Correlating Two Loaded Images.

Reset

Deletes all correlation points in the images.

Create Correlation

Active only if all correlation points are set in both images.

Creates a correlative overlay image. A third image container with the correlated image will be opened in the Center Screen Area and the Show Correlation checkbox will be activated automatically.

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