Parameter

Description

Toolbar

Offers tools to draw regions of interest into the image. These regions are then used for analysis.

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Select

Activates the selection mode that enables you to select the graphic elements in the image area.

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Clone

Activates the cloning mode that you can use to create an identical copy of the last graphic element drawn in by simply clicking anywhere into the image area. To exit this mode, either switch back to the selection mode or press the ESC key.

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Draw Rectangle

Enables you to draw a rectangular region of interest (ROI) into the image.

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Draw Circle

Enables you to draw a circle.

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Draw Ellipse

Enables you to draw a elliptical region.

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Draw Spline Contour

Enables you to draw a spline contour. You can either define the corner points by a series of clicks or you can trace a contour by keeping the left mouse key pressed. Close this contour by right-clicking. Corners are always rounded with this tool.

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Draw Polygon

Enables you to draw a polygonal region.

Parameter

Description

Method

Selects the analysis method. The FRET image is updated depending on the selected method.

For Sensitized Emission, ZEN provides three different methods for data analysis. The data table displays the results of all three methods.

Using the Acceptor Bleaching method is only possible with an input of a dual channel time series with a bleach event.

Save Image

Opens the FRET image as new image document.

ROI Table

Displays the list of regions that are drawn into the raw data image.

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#

Displays the identification number of the region.

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Type

Displays the type of the region.

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Object

Activated: Defines the region as one that should be analyzed.

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Background

Activated: Defines the region as background region.