For this step, a split view will appear. On the left side you see the Live image. On the right side you see the overview image with the defined ribbons.

To modify either the Live image or the image with the defined ribbons click on the corresponding container. The activated container will be marked with a white frame.

Again, like in step one for the overview image, you have to select the objective, channel, light source and adapt the exposure time. Additionally, you have to generate a focus surface to ensure that your sample will be in focus during the image acquisition.

If you click on the Create Ribbon Image button, the ribbon image will be acquired.