The following Bio Applications are currently available in ZEN:

• Cell Counting
• This module provides a simple automated image analysis workflow customized for counting of fluorescently labeled cell nuclei in biological samples and a tailored result presentation with interactive measurement tables, heatmap and plots. This module allows automatic monitoring of cell numbers and thus proliferation, i.e. under the influence of compound effects. The measurement features include the number and the density of the cell nuclei as well as the mean intensity and mean area of cells in a well.
• Confluency
• This module provides a simple automated image analysis workflow customized for quantifying the cell confluency using variance-based segmentation and a tailored result presentation with interactive measurement tables, heatmap and plots. Applications addressed by this module include cell confluency assays as a measure of quality control in cell-based assays and wound healing assays to follow cell migration and cell-cell interaction. The measurement features include the covered area and the area percentage.
• Gene- and Protein Expression
• This module provides a simple automated image analysis workflow customized to quantify gene expression and a tailored result presentation with interactive measurement tables, heatmap and plots. Applications addressed by this module include:
• Automatic evaluation and quantification of the transfection efficiency to, for example, optimize the transfection protocol or to pick positive clones for targeted assays
• Measurement of the expression level to quantify the abundance and distribution of labeled molecules within a cell population
• Quantification of viral or bacterial infection
• The measurement features include the total number of cells and number of positive cells, the percentage of positive cells and the mean intensity of the transfection channel.
• Automated Spot Detection
• This module provides a simple automated image analysis workflow customized to quantify spots in the cell nuclei (e.g. for FISH, telomeres, centromeres, foci counting,...) and a tailored result presentation with interactive measurement tables, heatmap and plots. The measurement features include total number of spots, average number of spots per cell, mean intensity of spots, mean area of nuclei, mean intensity of nuclei (nuclear stain).
• Translocation
• This module provides a simple automated image analysis workflow customized to automatically measure the translocation ratio between the cytoplasm and the nucleus and a tailored result presentation with interactive measurement tables, heatmap and plots. The measurement features include the total number of cells, the translocation ratio, as well as the mean intensity values of the nucleus and the ring.