1. You have set up the image analysis setting with the method ZOI (Zones of Influence) and have opened the Image Analysis Wizard, see Creating a New Image Analysis Setting. This has created the classes ZOIs/ZOI, Primary Objects/Primary Object, and Rings/Ring by default.
  1. If you want to extend the predefined list of classes, click Add Subclass in the Classes step.
  2. You can find an example how to detect objects on the ring in Counting the Number of Objects in a Ring around the Nucleus.
  3. If necessary, click Add Class to extend the predefined list by another independent class of objects.
    Note that you cannot add further rings.
  4. Select the image channel which you want to use for object detection. In this example, the primary objects (the nuclei) are in the AF568 channel. Therefore, click on the class Primary Object and select the channel containing the nuclei.

Parameter

Description

ZOIs

Class of all zones of influences.

ZOI

Individual zone of influence.

Primary Objects

Class of all primary objects.

Primary Object

Individual primary object.

Ring

Individual ring.

Ring Segment

Part of a ring (a ring often consists of only one ring segment).