1. To calculate ratios (quotient of two fluorescence intensities) and display ratio images, you need the Physiology (Dynamics) module.
2. You have a suitable image date set open.
3. You are in the MeanROI View on the Ratio tab (view option).

1. In the Method dropdown list select the Dual Wavelength or any other of the Ratio Type formulas present. entry.
2. In the Calculation dropdown lists select the channels for calculating the ratio.
3. The ratio values are calculated automatically.
3. Click on the Cache ratio image button to cache all the ratio images of the current time series with the given ratio calculation parameters.
4. The ratio values are calculated. The ratio image and a ratio diagram are displayed on the MeanROI view. For very large images (pixels and time points) it might be necessary to use the Cache Ratio Image function on the Ratio tab, as this will eliminate flickering when playing through the images at speed.

1. You have successfully calculated a ratio for a dual wavelength dye such as Fura-2.