1. Click on Live in the Acquisition tab to activate the live view in the Center Screen Area.
2. Navigate the stage manually to the first calibration marker on the sample holder (marked with No. 1) by means of the joystick. It is enough if you move the stage to the larger L-shaped calibration marker. The smaller marker will be detected automatically within the Sample Holder Calibration Wizard. To locate the marker positions we recommend using a dry objective with low magnification (5x – 20x).
   
3. Open the CAT tool.
4. Click on Calibrate… to open the Sample Holder Calibration Wizard.