Gordon et al., Biophys J 74, 2702 (1998)

FRETN = Fcorr/(Dcorr*Acorr)

Displays the FRET image with intensities converted from the FRET index calculated for each pixel using the Gordon method. This method calculates a corrected FRET value and divides by concentration values for donor and acceptor. This method attempts to compensate for variances in fluorochrome concentrations but overdoes it. As a result, cells with higher molecular concentrations report lower FRET values.