This tab is only available if you have licensed the Physiology (Dynamics) module.
ROI tracing allows you to adjust the position of your ROI as necessary to accommodate the lateral movement of an object in an image of which the mean intensity is to be measured. This is done by defining a series of one or more so called key frames for individual ROIs. In this manner, complex object movements can be corrected.

Parameter

Description

Enable ROI Tracing

Enables the functionality for ROI tracing.

Selected ROI

Displays the number and shape of the currently selected ROI.

Key Frame Edit Mode

Only available if you have selected a ROI.

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All Key Frames

Manipulates the ROI for all time points/key frames.

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Single Key Frame

Manipulates the ROI for the currently selected time point and creates a key frame. Note that a single key frame adjustment can only be performed when the frame number (time point) is set to 2 or higher.

Interpolation

Only available if you have selected a ROI.
Selects an interpolation method for the ROI changes between the time points.

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Constant

Does not interpolate the ROI position between the key frames, i.e. the ROI is only present at the set key frames.

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Linear

Determines the ROI position at the time points between key frames based on linear interpolation.

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Spline

Determines the ROI position at the time points between key frames based on spline interpolation.

Key frame list

Displays the key frames of the ROI and the changes compared to the previous key frame.

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Add

Adds the current position as key frame.

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Delete

Deletes the currently selected key frame.

Show

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Trajectories

Activated: Displays the trajectories between the key frames in the image.

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Ticks

Activated: Displays ticks for the (center) position the ROI for each time point in the image. These ticks are only visible if Linear or Spline is selected as interpolation method.

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Ghosted key frames

Activated: Displays the shape of the ROI at each key frame.
Deactivated: Displays the shape of the ROI only at the currently selected time point.

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All ghosted

Only available if Ghosted key frames is activated.
Activated: Displays the shape of the ROI for all time points. This effect is only visible if Linear or Spline is selected as interpolation method.