1. You are in the MeanROI View or in the MeanROI Setup on Acquisition tab.
  1. Go to the Graphics tab in the View Options.
  2. Select a tool for drawing in ROIs, e.g. the Polygon tool.
  3. Activate the Keep tool checkbox.
  4. The selected tool remains active after you have drawn in an ROI. This means you can draw in several ROIs without having to re-select the tool.
  5. Using the selected tool, in the image view draw in the objects or regions (ROIs) for which intensity measurements are required.
  6. The ROIs are displayed in the list (Annotations/ Measurements Layer) on the Graphics tab.
  7. Intensity measurements are performed for each ROI and displayed in the chart area to the right of the image view.
  1. You have successfully defined measurement regions for the intensity measurement.

Measurement time

Note that the time taken to initially create measurements will vary as some date is cached to memory. Thus, when a long time series image is opened that already contains ROIs, you might have to wait briefly until ZEN completes its measurements. The duration depends on e.g. number of ROIs, number of time points, image size, number of pixels etc.