This workflow does not work with LSM systems.

  1. Open the Channels tool in the Acquisition Parameter group.
  2. From the drop-down list, select the channel.
  3. The Add Dye or Contrast Method dialog opens.
  4. Select the desired dye or contrast method. You can search for a dye by entering its name (or starting letter) in the Search input field.
  5. Double-click the entry.
  6. The channel is added to your experiment. To add more channels, repeat the last 2 steps.
  7. Close the dialog.
  8. The added channels are displayed in the Channels tool.
  9. Click Set Exposure at the top of the Acquisition tab.
  10. The exposure time is now measured for all active configured channels one after the other. It will be also adopted into the settings for the channels. To set the exposure time channel specific, use the Set Exposure button in the channel settings of the channels tool.
  11. To save the experiment together with all the settings, go to the Experiment Manager and click .
  12. Options are displayed.
  13. Select the Save entry in the dropdown list.
  1. You have set up the multichannel experiment using the Channels tool, executed it and then saved the configuration. This means that you can repeat the experiment as often as you like using the same settings.