The analysis with the Sensitized Emission methods (all three methods are analyzed) shows the following parameters in the data table:

Parameter

Description

Region

Displays the identification number of regions assigned for analysis. The region zero refers to the whole image.

Time

Displays the time point if a time series is analyzed.

D avg.

Displays the average intensity of the region in the donor channel. These values are influenced by the selected settings in the Settings tab. They can vary from the values of the same regions in the Histo view.

A avg.

Displays the average intensity of the region in the acceptor channel. These values are influenced by the selected settings in the Settings tab. They can vary from the values of the same regions in the Histo view.

F avg.

Displays the average intensity of the region in the FRET channel. These values are influenced by the selected settings in the Settings tab. They can vary from the values of the same regions in the Histo view.

FRETN (p)

Displays the result of the analysis with the Gordon method. The FRETN value is calculated for each pixel, afterwards the average for the selected region is determined.

Fc (p)

Displays the result of the analysis with the Youvan method. The Fc value is calculated for each pixel, afterwards the average for the selected region is determined.

N-FRET (p)

Displays the result of the analysis with the Xia method. The N-FRET value is calculated for each pixel, afterwards the average for the selected region is determined.