Before you can work with the CAT module, you have to check the following settings on the light microscope system (hardware and software settings). In general, the system is calibrated by a service technician but we recommend checking the settings again, especially when you have changed components, e.g. objectives or filter cubes. As these general settings are not described here in detail, please ask your service technician, or read the ZEN Online Help.

• Check Parcentricity and Parfocality
  Note that the calibration of parcentricity and parfocality has to be done at the TFT display of the microscope.
• Check Camera Orientation and Stage Movement
  Before checking the stage movement the correct camera orientation always has to be set first. The camera orientation should match your view through the ocular of the microscope. If this is not the case, you can change the camera orientation in the Camera tool.
  To check the stage movement we recommend acquiring a tiles image and check if the tiles are put together correctly. If this is not the case, you have to close ZEN software and open the MTB 2011 software. There you must change the stage inversion in the configuration list under Motorized Stage.
• Perform Shading Correction
  Before starting the CAT workflow a shading correction has to be performed in ZEN software. Please read the corresponding chapter in the ZEN Online Help.