In this mode you can manually record a new image by pressing the F12 key on your keyboard. With this you can achieve different time intervals between the individual acquisitions when you change the focus position.

  1. You have opened the Manual Extended Depth of Focus tool on the Locate tab.
  1. If you want to store all acquired images in memory for a complete recalculation of the EDF image after each single acquisition, activate Z-Stack. Note that the more images you acquire, the more time it takes to calculate the EDF.
  2. Click F12 Key.
  3. Click Start to acquire the images.
  4. The Central Screen Area is split into two parts. On the left you can see the current live image.
  5. Press F12 on your keyboard to acquire an image manually.
  6. The calculated extended focus image on the right side is updated.
  7. Move to a new focus position.
  8. Repeat the last two steps until you have sharp images from all desired areas of your sample.
  9. Click Stop to finish your acquisition. If you want to pause the acquisition, click Pause.
  1. You have finished the acquisition of the manual EDF image. The resulting image is displayed in the Central Screen Area.