Data Table Acceptor Bleaching
The analysis with the Acceptor Bleaching method shows the following parameters in the data table:
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Parameter |
Description |
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|---|---|---|
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Region |
Displays the identification number of regions assigned for analysis. The region zero refers to the whole image. |
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|
FRET(p) Eff. % |
Displays the FRET efficiency for each pixel. It is calculated and averaged for all pixels of the region. The averaged intensities of the region are used to calculate an averaged FRET efficiency for this region. Delta D/D Post * 100. |
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|
D Pre |
Displays the average donor intensity of the region in the pre-bleach image. |
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|
D Post |
Displays the average donor intensity of the region in the post-bleach image. |
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A Pre |
Displays the average acceptor intensity of the region in the pre-bleach image. |
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A Post |
Displays the average acceptor intensity of the region in the post-bleach image. |
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Delta D |
Displays the change in donor intensity of the region before and after the bleach event. Delta D = D Post-D Pre. |
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Delta A |
Displays the change in acceptor intensity of the region before and after the bleach event. Delta A = A Post- A Pre. |
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