Only visible for multichannel fluorescence images.

The channels that you are comparing with one another are displayed in the image area in the form of a color overlay. The channel color of the image is used here. If the images have more than 2 channels, you can add additional channels on the Dimensions tab. This temporary selection is deactivated, however, when you select the channels for comparison on the Coloc. Tools tab.

In the Colocal. (Colocalization) view, you can analyze the extent of colocalization quantitatively in two fluorescence channels. The view consists of two main areas: the X/Y scatter plot on the left and the actual image (2 channels are displayed) in the right image area. Using the Coloc. Tools tab, you can also display the colocalization table in the lower image area.

The analysis is performed on regions drawn into the image. Once a region is drawn, it is automatically treated as an active region. The scatter plot shows the pixel value frequencies for this region.

The Colocalization table displays the data for the entire image and for the selected region. To select several regions, press the Ctrl key while clicking on the desired regions.

Apart from drawing regions into the image, you can also draw them into the X/Y scatter plot. If you have used the function in the regions section of the Coloc. Tools tab, only those pixels that are framed by a region in the scatter plot are taken into consideration. This means that you can correlate interesting point clouds quickly with the corresponding pixels in the image.