1. Click on the DAPI Individual Nuclei entry in the list.
  2. Click on the Edit button in the Region Features section.
  3. The Feature Selection dialog is opened.
  4. Double-click on Intensity Mean Value of channel "DAPI" and Intensity Mean Value of channel "mRFP1" features, one after another.
  5. The features are displayed in the Selected Features list on the left.
  6. Remove superfluous features (e.g. Area, Perimeter) from the list. Select the feature and click on the Delete button .
  7. Activate the Display checkbox for the features.
  8. Click on OK.
  9. The selected features are displayed in the Region Features section.
  10. In the section Annotation Options, activate the checkbox Color.
  11. Select Yellow from the drop-down list.
  12. Click on Next.