Step 6: Features
- Click on the DAPI Individual Nuclei entry in the list.
- Click on the Edit button in the Region Features section.
- The Feature Selection dialog is opened.
- Double-click on Intensity Mean Value of channel "DAPI" and Intensity Mean Value of channel "mRFP1" features, one after another.
- The features are displayed in the Selected Features list on the left.
- Remove superfluous features (e.g. Area, Perimeter) from the list. Select the feature and click on the Delete button
.
- Activate the Display checkbox for the features.
- Click on OK.
- The selected features are displayed in the Region Features section.
- In the section Annotation Options, activate the checkbox Color.
- Select Yellow from the drop-down list.
- Click on Next.
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