Options

Mirror Image

Here you can mirror the image horizontally or vertically by using the two buttons at the right. The alignment of the images depends on the microscope (upright/inverted) and orientation of the sample holder.

Keep tool

Activated: Keeps the current tool active. That is helpful if you want to draw in more than one ROI/POI.

Auto color

Activated: Uses a new color for each new element which is drawn in.

Snap to Pixel

Activated: Draws in graphical elements using the pixel grid.

Use fine calibration value

Activated: Uses the measured fine calibration.

The precision of relocation and therefore the quality of the overlay image can be improved by determination of an offset value. This value describes the offset between the loaded image and the live image. The defined offset value is only valid for the loaded image which you can see in the container. If another image is loaded or if you close the dialogue, the offset value will be deleted.
Determine the offset by identification of a POI (Point Of Interest) within the snapped image. To identify a POI use the buttons in the Regions section. By clicking on the Set Offset button, the stage moves to the supposed sample position. Compare the sample position within the live image with the set POI and correct the stage in that way that both shown positions are identically. Confirm the fine calibration with the OK button. Now the fine calibration is measured and the checkbox is activated.

More information, see Fine Calibration of the Sample Holder.

Double click in image to move stage

Activated: Moves the stage to the position you have double clicked on.

Refocus after stage movement

Activated: Adjusts the focus automatically after the stage has moved.

Move stage in z-direction before x/y movement

Activated: Moves the stage to the load position before it moves to the next correlative calibration marker.

Show splitter view

Activated: Activates Splitter Mode in the Center Screen Area.