When the registration for the data set is already determined and the transformation matrix is saved as an .xml file (Filename.czi.xml), this option can be selected.

Parameter

Description

Parameter Settings

Setting of alignment parameters.

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Select a registration file […]

Opens the Windows File Explorer to select a file.

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Dual side fusion

Merges the image done with the light sheet from the right side with the left side image. See also Dual Side Fusion.

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Fusion Subset in X

Activated: Selects which area of the left and the right illumination image should be used for fusion. Only available for Mean or Maximum fusion. See also the description in Dual Side Fusion.

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Expand to maximum value

Activated: The volume of the resulting data set after registration and fusion is determined and additional black pixels (x,y) and z-slices are added to the views during registration. This prevents loss of data for later fusion.

Parameter

Description

Input settings

A subset of a present time series can be selected for the Registration and Fusion processing.

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Processing Subset Time

Truncates time points.

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Processing Subset Steps

Defines time-to-time point step size.

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Scaling correction (µm)

Sets the geometric scaling between X/Y and Z.

Fusion

Fuses registered views in one z-stack or one z-stack over time.

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None

No fusion is performed after Registration and Dual Side Fusion.

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Mean Fusion

The pixel in the fused image is determined by averaging the intensity level of the pixels for the involved views.

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Mean Fusion + Multiview DCV

Only available if the dataset has not undergone deconvolution before.
The pixel in the fused image is determined by averaging the intensity level of the pixels of the involved views. After fusion, a deconvolution is performed. The Deconvolution interface is displayed if selected.

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Blending

Controls the amount of Blending (smoothing) by fading the borders of all z-stacks in order to prevent visible edge effects.
Note: The value for Blending should be as low as possible with typical values from 20 to 80. This needs to be tested for every dataset.

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Fusion Subset in Z

Activated: Defines which portion of the z-stacks of the contributing views of the experiment are used for the fusion process.

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Current View

Selects the current view. For each view, the z-range can be set via slider, spin boxes, or mouse click in the image. Registration will not be affected by the z-range settings.
Note that for later deconvolution the calculated point spread function (PSF) of a Multiview experiment assumes that all views equally contribute to all areas of the image. This is not the case when using Subset Z and might result in artefacts of the deconvolution result.

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Save unfused data

Activated: Saves the registered image in addition to the fused image.