This step is basically used for re-acquiring images from ROIs that are out of focus.

  1. You are in step 7/7 Reshoot.
  1. Check if the Select Tiles mode is activated. This mode should be selected by default when entering this step.
  2. You can use the arrows on each side of the image to navigate through the ROI series or the Z-Position slider on Dimensions tab.
  3. Select an image you want to re-acquire by clicking on it with the left mouse.
  4. The image will be displayed with a dashed green frame.
  5. Continue with checking the images and selecting the images you want to re-acquire.
  6. When you have selected all images to re-acquire click on Acquire.
  7. The software will move to the first image which you have selected.
  8. Adjust the focus and click on Snap.
  9. Click on Replace to replace the old image with the new one.
  10. The software will automatically move to the next selected image to be re-acquired.
  11. Continue until you have re-acquired all selected images.
  12. Click on Finish.
  1. You have successfully completed the Acquisition Wizard for the light microscope images of your ribbons. Continue with the process described in the next chapter of this guide.